Off-line monitoring of important parameters is a time consuming procedure due to manual nature of collecting information from multiple analyzers, and data entry into the controller of the fermenter. This information is important for the realization of a biotechnological process and for understanding the impact of cultivation conditions on efficiency, productivity and product quality. Other parameters such as cell viability, dissolved gases, nutrients, osmotic pressure, and cellular metabolites are monitored as indicators of reproducibility of culture. For example, the flow into the fermenter, the nutrient medium is carried out according to the results of the analysis of the culture medium. Some of these parameters are used in the process of operation of the fermenter. Other important parameters of the fermentation, are still typically monitored via manual sampling and using several off-line analyzers or routine methods of analysis. The possibility of controlling these parameters allows to carry out reliable, reproducible cycles of fermentation, and also scale biotechnological processes. As a rule, parameters such as pH, dissolved oxygen and temperature can be monitored by means of sensors of direct action (which are part of the fermenter/bioreactor). Monitoring of Cell cultures is essential for development, optimization and management of productive and sustainable cultivation processes. In addition, the BioProfile FLEX analyzers have the ability to set the feedback system with the controller of the fermenter, allowing to program, for example, the flow of feed not only on the content of oxygen in the culture medium, but also on the concentration of glucose or other nutrient concentrations of the substrates.įermentation-based mammalian cell culture is widely used for industrial production of therapeutic proteins. The automation of monitoring processes increase the biomass of cultures of cells allows to obtain more reliable results that will not depend on the operator. The measurement process takes place online without any interruption/disturbances of fermentation. The results of studies of mammalian cell culture during the fermentation process show that the system significantly reduces manual labour and associated measurement errors. The application of the new integrated multi-function analyzer BioProfile FLEX automated sampling system
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
December 2022
Categories |